FTIR-Based Metabolomics for Characterization of Antioxidant Activity of Different Parts of Sesbania grandiflora Plant

Sesbania grandiflora, one of the flowering plants with great potential as a source natural antioxidants because it contains chemicals such tannin, phenolics, and flavonoids. However, there has been no extensive investigation on antioxidant activity isolated from different parts this plant. This study aims to investigate correlation between secondary metabolites extracted three (leaves, stem barks, roots) S. grandiflora plant using Fourier-transform infrared spectroscopy (FTIR) based metabolomics approach. The FTIR is very useful technique for identifying functional groups present in mixtures, while assay provides base select part most antioxidant. properties were determined 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), potassium ferricyanide reduction method. multivariate data - analyses Principal Component Analysis (PCA) Partial Least Square (PLS) conducted compare distribution investigated. PLS was performed evaluate relationship components extracts obtained their activities. results exhibited that activities extract roots are higher than leaves. Also, model indicated group absorption significantly correlated IC50 values activity. Subsequently, ​​analysis displayed C=C, C=O, along C-O proposed main contributors tested. grouped PCA analysis total principal (PC) 94%.